lumiprobe荧光染料介绍

发表于2016年1月8日由jinpanbio

Lumiprobe荧光染料

Lumiprobe荧光染料是美国一家高品质生物技术公司，专业提供分子生物学研究用的活性荧光染料。主要产品有：活性染料（Reactive Dye）和SYBR Green I 染料，定制合成荧光双标记引物。产品主要应用：点击化学（Click Chemistry）、蛋白质组学研究中的双向荧光差异凝胶电泳（2D DIGE）和实时荧光定量PCR（Realtime PCR）。

Lumiprobe Corporation是美国一家高品质生物技术公司，专业提供分子生物学研究用的活性荧光染料。从2006年开始，公司生产并销售生命科学研究和诊断学应用的优质化学药品。产品主要有：活性染料(Reactive Dye)和SYBR Green I 染料，用于寡核苷酸合成的亚磷酰胺，点击化学用染料和其它试剂。产品主要应用：点击化学(Click Chemistry)、蛋白质组学研究中的双向荧光差异凝胶电泳(2D DIGE)和实时荧光定量PCR(Realtime PCR)。

作为Lumiprobe官方授权代理，上海金畔生物生物可提供Lumiprobe的完整产品线，满足您的各种科研实验需求。产品包括：羧酸类染料、羰基类染料，标记分子氨基活性染料，标记分子巯基活性染料，动物活体成像用染料，蛋白质、多肽、核酸等生物分子标记染料、点击化学等。渠道正规（市场上部分是分装，质量不能保证），质量保证，价格极具竞争力,而且我们的货期仅需要3-5工作日，不需要像其他家攒单货期3-4周。

Lumiprobe染料主要用途:

NHS ESTER 适合标记氨基酸

azide 适合标记寡核苷酸和DNA
maleimide 适合标记有半胱氨酸的蛋白和多肽以及其他巯基化分子
alkyne 适合标记寡核苷酸和DNA
hydrazide 适合标记醛和酮
carboxylic acid 非活化的

lumiprobe荧光染料分类：

Dye NHS esters

Dye azides

Dye alkynes

Dye maleimides

Dye hydrazides

Carboxylic acids

Amino dyes

Tetrazines

Sulfo-Cyanine3 tetrazine

Cycloalkyne dyes

Cyanine5 DBCO

Miscellaneous dyes

BDP TR methyl ester, 5 mM in DMSO

Click Chemistry reagents

Non-fluorescent azides

Azidobutyric acid NHS ester
Biotin azide

Non-fluorescent alkynes

Alkyne NHS ester (hexynoic acid NHS ester)
Alkyne hydrazide
Alkyne maleimide
Biotin alkyne
DBCO NHS ester
EdU (5-ethynyl-2′-deoxyuridine)
Pentynoic acid STP ester

DNA

PCR/qPCR

Amino-11-dUTP
Amino-11-ddUTP
ROX reference dye for qPCR
dsGreen Gel Staining Solution, 10000×
dsGreen for Real-Time PCR, 100×

Modified triphosphates

Amino-11-dUTP
Amino-11-ddUTP

Phosphoramidites

CPG solid supports

Alkyne CPG modifier 500
Phosphate CPG solid support
TFA-Amino modifier CPG 500

Miscellaneous reagents

Bifunctional crosslinkers

Alkyne NHS ester (hexynoic acid NHS ester)
Alkyne hydrazide
Alkyne maleimide
Azidobutyric acid NHS ester
DBCO NHS ester
Pentynoic acid STP ester
Sulfo-Cyanine5 bis-NHS ester

Auxiliary reagents

Ascorbic acid
Copper(II)-TBTA complex, 10 mM in 55% aq. DMSO
DMF (dimethylformamide), labeling grade

Proteomic reagents

3Dye™ 2D DIGE Kits
Cyanine2 NHS ester minimal dye
Cyanine3 NHS ester minimal dye
Cyanine5 NHS ester minimal dye

Lumiprobe 水溶性cy染料

发表于2016年1月8日由jinpanbio

Lumiprobe 水溶性Cy染料

磺化和非磺化染料都可用于标记生物分子例如DNA和蛋白质。染料之间的差异是它们的溶解性：磺基染料是水溶性的，并且它们不使用有机共溶剂用于在水性环境中的标记。它们不易于在水中聚集。

There are two varieties of cyanine dyes: non-sulfonated cyanines, and sulfonated cyanines. For many applications they are interchangeable, because their spectral properties are nearly identical. Both sulfonated and non-sulfonated dyes can be used for the labeling of biomolecules such as DNA and proteins. The difference between the dyes is their solubility: sulfo- dyes are water-soluble, and they do not use of organic co-solvent for the labeling in aqueous environment. They are less prone to aggregation in water.

Sulfo-Cyanine3 carboxylic acid
Sulfo-Cyanine5 carboxylic acid
Sulfo-Cyanine5.5 carboxylic acid
Sulfo-Cyanine7 carboxylic acid
Sulfo-Cyanine7.5 carboxylic acid

磺酸基-Cy5羧酸

无活性的磺酸基-Cy5羧酸，水溶性染料。此染料是高水溶性染料
用于分子标记应该选择具有活性的磺酸基-Cy5 NHS酯

分子结构：

一般性质：
外观：深蓝色粉末
分子量：664.76
分子式：C32H37N2NaO8S2
溶解性：易溶于水，DMSO,DMF，在多数有机溶剂难溶
质控：NMR 1H（95%）和13C,TLC

光谱性质：
最大激发波长：646nm
在最大激发波长下的消光系数：271000Lmol-1cm-1
最大发射波长：662nm
荧光量子产率：0.2

General properties

Appearance:	dark blue powder
Molecular weight:	664.76
CAS number:	1121756-16-8, 1144162-77-5
Molecular formula:	C₃₂H₃₇N₂NaO₈S₂
Solubility:	Good in water, DMF, DMSO. Low in non-polar organic solvents.
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

sulfo-Cyanine5 carboxylic acid structure

sulfo-Cyanine5 absorbance and emission spectra

Cat. #	Quantity	Lead time
13390	1 mg	in stock
23390	5 mg	in stock
43390	25 mg	in stock
53390	50 mg	in stock
63390	100 mg	in stock

磺酸基-Cy7羧酸

磺酸基-Cy7羧酸是无活性易溶于水的近红外染料；
此试剂不能用于标记分子，它有较高的水溶性，可改善近红外光谱范围的量子产率,并具有很高的消光系数。

分子结构：

一般性质：
外观：深绿色粉末
分子量：730.87
分子式：C37h33N2NaO8S2
溶解性：易溶于水，DMF,DMSO
质控：HPLC（95%），NMR 1H 和13C

光谱性质：
最大激发波长：740nm
在最大激发波长下的消光系数：240600Lmol-1cm-1
最大发射波长：773nm

General properties

Appearance:	dark green powder
Molecular weight:	730.87
Molecular formula:	C₃₇H₄₃N₂NaO₈S₂
Solubility:	Good in water, DMF, DMSO. Low in non-polar organic solvents.
Quality control:	NMR ¹H, HPLC (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.

Sulfo-Cyanine7 carboxylic acid is non-reactive water soluble near infrared dye.

The reagent is useful as a fluorescent marker in NIR range when attachment to other molecules is not desired. It has high hydrophilicity and aqueous solubility, improved quantum yield in NIR range, and very high molar extinction coefficient.

This reagent contains free carboxylic acid function. Pre-activated activated ester – sulfo-Cyanine7 NHS ester is also available.

sulfo-Cyanine7 absorbance and emission spectra

15390	1 mg	in stock
25390	5 mg	in stock
45390	25 mg	in stock
55390	50 mg	in stock
65390	100 mg	in stock

磺基-Cy3 NHS酯：

此染料可溶于水，氨基活性Cy3染料。在水溶液中有效标记蛋白和多肽，无需共溶剂，是低溶解性蛋白和易于降解蛋白的理想选择。
此染料是磺化，亲水性染料。可以代替Alexa Fluor 546和DyLight 549

分子结构：
一般性质：
外观：深红色晶体
分子量：735.8
分子式：C34H38N3NaO10S2
溶解性：极易溶于水，易溶于有机溶剂（DMF,DMSO）
质控：NMR 1H（95%）和13C,TLC，功能性测试

光谱性质：
最大激发波长：548nm
最大激发波长下的消光系数：162000Lmol-1cm-1
最大发射波长：563nm
荧光量子产率：0.1

General properties

Appearance:	dark red crystals
Molecular weight:	735.80
Molecular formula:	C₃₄H₃₈N₃NaO₁₀S₂
Solubility:	very high in water, good in polar organic solvents (DMF, DMSO)
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Water soluble sulfo-Cy3 NHS ester structure

sulfo-Cyanine3 absorbance and emission spectra

11320	1 mg	in stock
21320	5 mg	in stock
41320	25 mg	in stock
51320	50 mg	in stock
61320	100 mg	in stock

磺基-Cy5 NHS酯：

水溶性Cy5 NHS酯，用于标记生物分子的氨基，无需任何共溶剂，因此可以用于标记在有机溶剂中易降解的蛋白和溶解性低的蛋白
Cy5 是最常用的荧光团之一，与多种设备兼容，比如平板读数仪，显微镜和成像仪
此染料是高水溶性，可以代替多种用途的Alexa Fluor 647,DyLight 649

分子结构：
一般性质：
外观：深蓝色粉末
分子量：761,84
分子式：C36H40N3NaO10S2
溶解性：极易溶于水，易溶于有机溶剂DMF和DMSO
质量控制：NMR 1H （95%）和13C,TLC,功能性测试

光谱性质：
最大激发波长：646nm
最大激发波长下的消光系数：271000Lmol-1cm-1
最大发射波长：662nm
荧光量子产率：0.2

General properties

Appearance:	dark blue powder
Molecular weight:	777.95
Molecular formula:	C₃₆H₄₀N₃NaO₁₀S₂
Solubility:	very good in water, good in DMF and DMSO
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

sulfo-cy5-nhs-ester

Cy5 NHS ester structure

sulfo-Cyanine 5 absorbance and emission spectra

13320	1 mg	in stock
23320	5 mg	in stock
43320	25 mg	in stock
53320	50 mg	in stock
63320	100 mg	in stock

磺基-Cy7 NHS酯：

一般性质：
外观：深绿色粉末
分子量：827.94
分子式：C41H46N3NaO10S2
溶解性：易溶于水,DMF,DMSO
质控：NMR 1H(95%)13C,TLC,功能测试

光谱性质：
最大激发波长：740nm
在最大激发波长下的消光系数：240600Lmol-1cm-1
最大发射波长：773nm

General properties

Appearance:	dark green powder
Mass spec M+ increment:	868.2
Molecular weight:	869.10
Molecular formula:	C₄₃H₄₉KN₄O₉S₂
IUPAC name:	1-[2-(6-{2-[(E)-2-[(E)-3-{(E)-2-[1-Methyl-3,3-dimethyl-5-(oxysulfonyl)-2-indolinylidene]ethylidene}-1-cyclohexen-1-yl]ethenyl]-3,3-dimethyl-5-(oxysulfonyl)-3H-indol-1-yl}hexanoylamino)ethyl]-1H-pyrrole-2,5-dione, potassium salt
Solubility:	Good in water, DMF, DMSO. Low in non-polar organic solvents.
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

sulfo-Cyanine7 maleimide structure

sulfo-Cyanine7 absorbance and emission spectra

15380	1 mg	in stock
25380	5 mg	5 days
45380	25 mg	in stock
55380	50 mg	in stock

Sulfo-Cyanine5 maleimide 磺基-cy5-马来酰亚胺

水溶的亲水的sulfo-Cyanine5 maleimide（Cy5® maleimide的类似物）。作为Cy5® maleimide的完美的替代物，我们推荐该产品用于蛋白、包括抗体的标记。通过凝胶过滤、离心柱纯化、透析、电泳或色谱层析方法就可以很容易的将标记蛋白和未标记的染料进行分类。
没有磺化基团的类似物：Cyanine5 maleimide。

磺酸基类荧光染料：

Sulfo-Cyanine3 NHS ester
Sulfo-Cyanine5 NHS ester
Sulfo-Cyanine5 bis-NHS ester
Sulfo-Cyanine5.5 NHS ester
Sulfo-Cyanine7 NHS ester

Sulfo-Cyanine3 maleimide
Sulfo-Cyanine5 maleimide
Sulfo-Cyanine7 maleimide

Sulfo-Cyanine3 azide
Sulfo-Cyanine5 azide
Sulfo-Cyanine5.5 azide
Sulfo-Cyanine7 azide

Sulfo-Cyanine3 alkyne
Sulfo-Cyanine5 alkyne
Sulfo-Cyanine5.5 alkyne
Sulfo-Cyanine7 alkyne

Sulfo-Cyanine3 amine
Sulfo-Cyanine5 amine
Sulfo-Cyanine5.5 amine
Sulfo-Cyanine7 amine

Lumiprobe 产品目录

发表于2016年1月8日由jinpanbio

Lumiprobe-Cyanine3.5 NHS ester

发表于2016年1月8日由jinpanbio

Lumiprobe-Cyanine3.5 NHS ester

Cyanine3.5 NHS ester（Cy3.5® NHS ester的类似物）是标记多肽、蛋白的氨基基团和寡核苷酸的活性染料。可以替代Cy3.5®, Alexa Fluor 594, DyLight 594。

一般性质

外观：深紫色粉末

分子量：690.27

分子式：C₄₂H₄₄ClN₃O₄

溶解性：溶于有机溶剂（DMF, DMSO,二氯甲烷），不溶于水。

储存条件：储存：收到货后于-20℃黑暗保存24个月。运输：室温3周，避免长时间暴露于阳光，干燥。

光谱性质

最大激发波长：591

在最大激发波长下的消光系数：116000

最大发射波长：604

荧光量子产率：0.35

Cy® is a trademark of GE Healthcare.

General properties

Appearance:	dark purple powder
Molecular weight:	690.27
Molecular formula:	C42H44ClN3O4
Solubility:	soluble in organic solvents (DMF, DMSO, dichloromethane), insoluble in water
Quality control:	NMR 1H and HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download

Spectral properties

Excitation maximum, nm:	591
Extinction coefficient at excitation maximum, Lmol-1cm-1:	116000
Emission maximum, nm:	604
Fluorescence quantum yield:	0.35

Cyanine3.5 NHS ester (an analog of Cy3.5® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides.

Cyanine3.5 NHS ester can replace NHS esters of Cy3.5®, Alexa Fluor 594, DyLight 594.

Lumiprobe-Cyanine3.5 NHS ester结构式：

订购货号	产品名称及规格
12020	Cyanine3.5 NHS ester, 1 mg
22020	Cyanine3.5 NHS ester, 5 mg
42020	Cyanine3.5 NHS ester, 25 mg
52020	Cyanine3.5 NHS ester, 50 mg
62020	Cyanine3.5 NHS ester, 100 mg

Lumiprobe-Cyanine5.5 NHS ester

发表于2016年1月8日由jinpanbio

Lumiprobe-Cyanine5.5 NHS ester

Cy5.5 NHS是近红外胺类活性染料。

CY5.5 NHS ester是用于标记多肽、蛋白和寡核苷酸的氨基基团的活性染料。CY5.5属于是近红外荧光染料，当背景荧光有干扰时适宜用它来做标记以进行荧光分析。它也可以用于活体成像实验。该染料可以替代Alexa Fluor 680和DyLight 680。

一般性质

外观：深蓝色粉末

分子量：716.31

分子式：C₄₄H₄₆ClN₃O₄

溶解性：溶于有机溶剂（DMSO,DMF,二氯甲烷），微溶于水。

质控：NMR 1H（95%）和13C，TLC，功能测试。

储存条件：储存：收到货后于-20℃避光保存24个月。运输：室温3周，避免长时间暴露于阳光，干燥。

光谱性质：

最大激发波长：673

在最大激发波长下的消光系数：209000

最大发射波长：707

荧光量子产率：0.2

During the last years, Cyanine5 (an analog of Cy5^®) has become an incredibly popular label in life science research and diagnostics. The fluorophore has its emission maximum in the red region, where many CCD detectors exhibit maximum sensitivity, and biological objects show low background. The dye color is very intense, therefore quantities as small as 1 nmol can be detected in gel electrophoresis by naked eye.

This Cyanine5 NHS ester (analog to Cy5^® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties.

Cy® is a trademark of GE Healthcare.

General properties

Appearance:	dark blue powder
Molecular weight:	716.31
Molecular formula:	C44H46ClN3O4
Solubility:	soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
Quality control:	NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download

Spectral properties

Excitation maximum, nm:	673
Extinction coefficient at excitation maximum, Lmol-1cm-1:	209000
Emission maximum, nm:	707
Fluorescence quantum yield:	0.2

订购货号	产品名称及规格
17020	Cyanine5.5 NHS ester, 1 mg
27020	Cyanine5.5 NHS ester, 5 mg
47020	Cyanine5.5 NHS ester, 25 mg
57020	Cyanine5.5 NHS ester, 50 mg
67020	Cyanine5.5 NHS ester, 100 mg

Lumiprobe Cy5 NHS 酯 Cyanine5 NHS ester

发表于2016年1月8日由jinpanbio

Cy5 NHS 酯 Cyanine5 NHS ester

在过去的一些年，Cy5荧光基因是生命科学研究和诊断领域非常受欢迎的标记试剂。在红色区域有最大的荧光发射，许多CCD检测器有最大灵敏性，并且背景低。染料颜色非常深，因此即使在凝胶上少至1nmol的量用肉眼也可观察到。

Cy5 NHS酯是用来标记多肽，蛋白和寡核苷酸氨基的活性染料。此染料只需少量的共溶剂（比如DMF或DMSO）来标记反应。此试剂用来高效标记可溶性蛋白，各种多肽和寡核苷酸的氨基。对于较复杂的靶标比如易降解的蛋白，不能用DMF或DMSO时，可以考虑用水溶性sulfo-Cyanine 5 NHS 酯不需要共溶剂，并且两者有非常相似的荧光特性。

Cy5荧光基因与各种设备兼容包括许多荧光显微镜，成像仪，扫描设备和荧光成像仪等。

Cy5 NHS酯可以替代Alexa Fluor 647, and DyLight 649和Cy3.5®活性酯。

分子结构

一般性质

外观：深蓝色粉末

分子量：616.19

分子式：C₃₆H₄₂ClN₃O₄

CAS号：1032678-42-4, 350686-88-3

溶解性：易溶于有机溶剂（DMSO,DMF和氯化物），水中的溶解性低。

质控：NMR 1H（95%）和13C，TLC, 功能测试

储存条件：储存：收到货后于-20℃避光保存24个月。运输：室温3周，避免长时间暴露于阳光，干燥。

光谱性质：

最大激发波长：646

在最大激发波长下的消光系数：250000

最大发射波长：662

荧光量子产率：0.2

General properties

Appearance:	dark blue powder
Molecular weight:	616.19
CAS number:	1032678-42-4, 350686-88-3
Molecular formula:	C₃₆H₄₂ClN₃O₄
Solubility:	good in polar (DMSO, DMF) and chlorinated (DCM, chloroform) organic solvents, low solubility in water
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Cat. #	Quantity	Price	Lead time
13020	1 mg	110.00$	in stock
23020	5 mg	210.00$	in stock
43020	25 mg	410.00$	in stock
53020	50 mg	695.00$	in stock
63020	100 mg	1190.00$	in stock

Product citations 参考文献

Li, L.; Sun, W.; Li, L.; Liu, Y.; Wu, L.; Wang, F.; Zhou, Z.; Zhang, Z.; Huang, Y. A pH-responsive sequential-disassembly nanohybrid for mitochondrial targeting. Nanoscale, in press. doi: 10.1039/c6nr07004c
Matsuo, Y.; Maurer, S.P.; Yukawa, M.; Zakian, S.; Singleton, M.R.; Surrey, T.; Toda, T. An unconventional interaction between Dis1/TOG and Mal3/EB1 promotes the fidelity of chromosome segregation. Journal of Cell Science. doi: 10.1242/jcs.197533
Stephen, Z.R.; Gebhart, R.N.; Jeon, M.; Blair, A.A.; Ellenbogen, R.G.; Silber, J.R.; Zhang, M. pH-Sensitive O6-Benzylguanosine Polymer Modified Magnetic Nanoparticles for Treatment of Glioblastomas. Bioconjugate Chemistry, in press. doi:10.1021/acs.bioconjchem.6b00545
Bloch, M.B.D.; Yavin, E.; Nissan, A.; Ariel, I.; Kenett, R.; Brass, D.; Rubinstein, A. The effect of linker type and recognition peptide conjugation chemistry on tissue affinity and cytotoxicity of charged polyacrylamide. Journal of Controlled Release, in press. doi:10.1016/j.jconrel.2016.06.038

Lumiprobe-Cyanine7 NHS ester

发表于2016年1月8日由jinpanbio

Lumiprobe-Cyanine7 NHS ester

胺类活性染料，近红外荧光染料，Cy7®的改进的类似物。

近红外荧光素可以被用来利用生物组织的近红外窗口。在这个光谱区域，高度增强的组织透明度使得活体成像成为可能。该染料可用于制备CY7标记的生物分子来进行后续试验，如活体研究及药物开发相关实验。

Cy7的特殊结构加固了中心聚亚甲基（polymethyne）链的稳定性。这种增强的分子结构会使其量子产率较其母结构提供20%，因此它的荧光强度会更大。
该试剂在标记时需要共溶剂有机溶剂。水溶Cy7 NHS ester可以用于蛋白NIR标记。

一般性质

外观：绿色粉末

分子量：682.29

分子式：C₄₁H₄₈ClN₃O₄

溶解性：溶于有机溶剂（DMSO,DMF,二氯甲烷），微溶于水。

质控：NMR 1H（95%）和13C，TLC，功能测试。

储存条件：储存：收到货后于-20℃避光保存24个月。运输：室温3周，避免长时间暴露于阳光，干燥。

光谱性质：

最大激发波长：750

在最大激发波长下的消光系数：199000

最大发射波长：773

荧光量子产率：0.3

Cy® is a trademark of GE Healthcare.

General properties

Appearance:	green powder
Molecular weight:	682.29
Molecular formula:	C41H48ClN3O4
Solubility:	soluble in organic solvents (DMSO, DMF, dichloromethane), low solubility in water
Quality control:	NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download

Spectral properties

Excitation maximum, nm:	750
Extinction coefficient at excitation maximum, Lmol-1cm-1:	199000
Emission maximum, nm:	773
Fluorescence quantum yield:	0.3

Lumiprobe-Cyanine7 NHS ester结构式：

Cy7 NHS ester structure

订购货号	产品名称及规格
15020	Cyanine7 NHS ester, 1 mg
25020	Cyanine7 NHS ester, 5 mg
45020	Cyanine7 NHS ester, 25 mg
55020	Cyanine7 NHS ester, 50 mg
65020	Cyanine7 NHS ester, 100 mg

Lumiprobe磺基cy5马来酰亚胺 Sulfo-Cyanine5 maleimide（水溶性）

发表于2016年1月8日由jinpanbio

Lumiprobe磺基cy5马来酰亚胺 Sulfo-Cyanine5 maleimide（水溶性）

水溶的亲水的sulfo-Cyanine5 maleimide（Cy5® maleimide的类似物）。作为Cy5® maleimide的完美的替代物，我们推荐该产品用于蛋白、包括抗体的标记。通过凝胶过滤、离心柱纯化、透析、电泳或色谱层析方法就可以很容易的将标记蛋白和未标记的染料进行分类。没有磺化基团的类似物：Cyanine5 maleimide。

Cy® is a trademark of GE Healthcare.

General properties

Appearance:	dark blue powder
Molecular weight:	803.00
Molecular formula:	C38H43KN4O9S2
Solubility:	soluble in water, DMSO, DMF
Quality control:	NMR 1H, HPLC-MS (95+%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.

Spectral properties

Excitation maximum, nm:	646
Extinction coefficient at excitation maximum, Lmol-1cm-1:	271000
Emission maximum, nm:	662
Fluorescence quantum yield:	0.2
CF260:	0.13
CF280:	0.13

磺基cy5马来酰亚胺 Sulfo-Cyanine5 maleimide（水溶性）结构式：

sulfo-Cyanine5 maleimide structure

磺基cy5马来酰亚胺 Sulfo-Cyanine5 maleimide（水溶性）订购信息：

订购货号	产品名称及规格
13380	Sulfo-Cyanine5 maleimide, 1 mg
23380	Sulfo-Cyanine5 maleimide, 5 mg
43380	Sulfo-Cyanine5 maleimide, 25 mg
53380	Sulfo-Cyanine5 maleimide, 50 mg
63380	Sulfo-Cyanine5 maleimide, 100 mg

Lumiprobe 磺酸基Cy 5活性脂 Sulfo-Cyanine 5 NHS ester

发表于2016年1月8日由jinpanbio

Lumiprobe 磺酸基Cy 5活性脂 Sulfo-Cyanine 5 NHS ester

水溶Cyanine 5琥珀酰亚胺酯（succinimidyl ester（SE）），类似于Cy5® NHS ester，不需要有机溶剂即在水相中即可对各种带氨基分子进行标记。因此该产品尤其适用于对那些在有机溶剂中易变性的蛋白和低溶解度蛋白的标记。
Sulfo-Cyanine 5是Cy5®的衍生物，与很多仪器如读板机（plate readers），显微镜和成像设备等相兼容。是最受欢迎的荧光染料之一。

该染料是高度亲水的、水溶的。

非磺化的衍生物：Cyanine 5 NHS ester。
可以在所有的应用中替代Cy5®，Alexa Fluor 647和DyLight 649。

一般性质

外观：深蓝色粉末

分子量：761.84

分子式：C₃₆H₄₀N₃NaO₁₀S₂

溶解性：极易溶于水，易溶于DMF和DMSO。

质控：NMR 1H(95%)和13C,TLC,功能性测试

储存条件：储存：收到货后于-20℃避光保存24个月。运输：室温3周，避免长时间暴露于阳光，干燥。

光谱性质

最大激发波长：646

在最大激发波长下的消光系数：271000

最大发射波长：662

荧光量子产率：0.2

General properties

Appearance:	dark blue powder
Molecular weight:	761.84
Molecular formula:	C₃₆H₄₀N₃NaO₁₀S₂
Solubility:	very good in water, good in DMF and DMSO
Quality control:	NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download

Spectral properties

Excitation maximum, nm:	646
Extinction coefficient at excitation maximum, Lmol-1cm-1:	271000
Emission maximum, nm:	662
Fluorescence quantum yield:	0.2
CF260:	0.13
CF280:	0.13

Sulfo-Cyanine5.5 NHS ester 磺酸基Cy5.5NHS（水溶性）

发表于2016年1月8日由jinpanbio

Sulfo-Cyanine5.5 NHS ester 磺酸基Cy5.5NHS

Sulfo-Cyanine5.5 NHS ester is an amine reactive activated ester of sulfonated far red Cyanine5.5 fluorophore, an analog of Cy5.5®. This is a reagent of choice for the labeling of antibodies, sensitive proteins, and others which require reactions in purely aqueous environment, or without any significant additions of organic co-solvents.

The dye is well suitable for non-invasive in vivo NIR imaging, and for the applications requiring low fluorescent background.

该染料是高度亲水的、水溶的。非磺化的衍生物：Cyanine 5 NHS ester。

17320	Sulfo-Cyanine 5 .5NHS ester, 1 mg	2080.00
27320	Sulfo-Cyanine 5 .5NHS r, 5 mg	5330.00
47320	Sulfo-Cyanine 5 .5NHS , 25 mg	11050.00
57320	Sulfo-Cyanine 5 .5NHS , 50 mg	19500.00
67320	Sulfo-Cyanine 5 .5NHS , 100 mg	31850.00

General properties

Appearance:	dark blue solid
Mass spec M+ increment:	1011.0
Molecular weight:	1128.41
Molecular formula:	C₄₅H₄₄K₃N₃O₁₆S₄
Solubility:	good in water, DMF, DMSO
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	Download

Spectral properties

Excitation maximum, nm:	675
Emission maximum, nm:	694

Product citations

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Abolmaali, S.; Tamaddon, A.; Kamali-Sarvestani, E.; Ashraf, M.; Dinarvand, R. Stealth Nanogels of Histinylated Poly Ethyleneimine for Sustained Delivery of Methotrexate in Collagen-Induced Arthritis Model. Pharmaceutical Research, 2015, 32(10), 3309–3323. doi:10.1007/s11095-015-1708-0