Hampton 银弹 Silver Bullets
Applications
| Additive screen for the optimization of protein crystals |
| For use with soluble proteins and membrane proteins |
| Additive screen to discover different crystal forms |
| Secondary or orthogonal crystallization screen when traditional screens are not successful |
| Additive screen for the optimization of protein solubility and stability |
Features
| Developed at Hampton Research | |||||||
Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization
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Description
Silver Bullets screens a portfolio of small molecules for their ability to establish stabilizing, inter molecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote stability, lattice formation, and crystallization.1-3
Published results with the Silver Bullets have been very encouraging, with more than twice as many proteins being crystallized overall as were crystallized from controls free of any small molecules.1-3
X-ray diffraction analysis has revealed the small molecule Silver Bullets in the crystal lattice, involved at the centers of hydrogen bonding networks and electrostatic interaction.1-3 Silver Bullets is compatible with hanging, sitting and sandwich drop vapor diffusion, microbatch, free interface, and microdialysis crystallization methods. Silver Bullets can be used with Dynamic Light Scattering (DLS), ThermoFluor, and Size Exclusion Chromatography assays.
Silver Bullets reagent portfolio
•Organic salts and acids
•Biologically active small molecules
•Amino acids and peptides
•Macromolecular digests
The Silver Bullets kit is a library of small molecules that have been shown to promote crystal lattice formation. X-ray diffraction analysis has demonstrated the reagents have the ability to:
•Stabilize the conformation of the protein
•Perturb the interaction of the protein with the solvent
•Participate in forming important lattice contacts
•Build the crystal lattice by forming reversible cross-links between the macromolecules in the crystal
The Silver Bullets kit is composed of 96 solutions in a single Deep Well block (Greiner 786261 block specifications: Total Volume: 0.5 mL Working Volume: 0.03 – 0.7 mL at Room Temperature 0.03 – 0.55 mL at -20ºC, Well Profile: Conical (V), Bottom Well Bottom: Solid, Plate Color: Translucent), HT format.
Each Silver Bullets reagent is a mixture of small molecules or macromolecular digests in 0.02 M HEPES sodium pH 6.8 buffer. Each solution contains between 2 and 20 small molecules.
Silver Bullets reagent volume is 0.5 ml (each well).
ThermoFluor is a registered trademark of Johnson & Johnson.
Hampton Silver Bullets Bio
Silver Bullets • Silver Bullets Bio
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Applications 应用
| Additive screen for the optimization of protein crystals |
| For use with soluble proteins and membrane proteins |
| Additive screen to discover different crystal forms |
| Secondary or orthogonal crystallization screen when traditional screens are not successful |
| Additive screen for the optimization of protein solubility and stability |
Features
Screens a portfolio of small molecules and excipients for their ability to establish stabilizing, intermolecular, hydrogen bonding, hydrophobic and electrostatic interactions which could promote lattice formation and crystallization
|
|||||||
| Developed at Hampton Research | |||||||
| Two sets of 96 reagents, composed of more than 1,090 chemicals, of which more than 400 are uniqu |
Please note, the Silver Bullets Bio Screen is temporarily unavailable as a new formulation is in development
GRAS reagent crystallization screen for proteins, including monoclonal antibodies, where Polyethylene glycol is the primary reagent, sampling pH 4.5 to 10