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Hampton蛋白结晶工具及仪器
Hampton蛋白结晶工具及仪器
CryoTong™ – Long
ApplicationsCrystal transfer under cryo temperature
FeaturesArtery clamp style closure30 seconds of cryo temperature180 mm length
Description
The CryoTong is a tool used to manually transfer a crystal mounted on a CrystalCap™ from liquid nitrogen to a magnetic base in a goniometer head positioned in a cryogenic stream, and then back to liquid nitrogen. The one-piece, compact CryoTong is available in two lengths. The long CryoTong is approximately 180 mm in overall length. The artery clamp style maintains the CryoTong in the closed position until the clamp is squeezed, which opens the opposing heads. The heads are non-magnetic stainless steel. The handle is magnetic stainless steel. The inside of the head is machined to closely surround the CrystalCap with loop and crystal in place. A small retaining lip is machined into the lower portion of the head to prevent the CrystalCap from slipping out of the CryoTong when the tool is in the closed position. The CryoTong can maintain the temperature of the crystal at -160°C for up to 30 seconds during room temperature crystal transfers. Choose the proper CryoTong size based upon the pin length required by the configuration of the x-ray data collection hardware used. The 18 mm CryoTong (HR5-112) is to be used with the CrystalCap HT and any other CrystalCap configured for 18 mm.HR5-114 Long CryoTong 18 mm, 180° features the head in alignment with the handle of the CryoTong.
Vial Clamp™ – Straight
ApplicationsVial support and manipulation
FeaturesStraight end tipHemostat style closureDescription
The Vial Clamp™ – Straight is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems™ straight when the clamp is closed. The clamp can be locked using the hemostat style closure. This clamp makes it easy to dip the storage vial into liquid nitrogen for crystal storage. The complete length of the clamp is straight, with an overall length of 195 mm. When the vial is placed in the clamp, the length of it is positioned perpendicular, 90° to the length of the clamp.
ApplicationsVial support and manipulation
FeaturesStraight end tipHemostat style closureDescription
The Vial Clamp™ – Straight is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems™ straight when the clamp is closed. The clamp can be locked using the hemostat style closure. This clamp makes it easy to dip the storage vial into liquid nitrogen for crystal storage. The complete length of the clamp is straight, with an overall length of 195 mm. When the vial is placed in the clamp, the length of it is positioned perpendicular, 90° to the length of the clamp.
CrystalWand™ Magnetic
ApplicationsCrystalCap Magnetic & CrystalCap Copper Magnetic handling toolDescription
The CrystalWand Magnetic is designed to be used exclusively with the CrystalCap Magnetic, CrystalCap Copper Magnetic, and CrystalCap HT systems during transfer of the caps from the vial to the goniometer head and from the goniometer to the vial. The 6 1/2” (165 mm) long chrome plated steel wand features a plastic housing enclosing a spring tensioned plunger that when depressed, moves a non-magnetic steel platform away from the magnet housed in the end of the wand. This causes the steel CrystalCap to detach from the wand and be placed readily into the vial or the CryoTong™. When the platform is retracted, the wand securely holds the steel CrystalCap Magnetic. The CrystalWand Magnetic 45° (McMiken Tool) offers the same functionality but is bent at a 45° angle at approximately 2.5 cm from the magnet end.HR4-729 CrystalWand Magnetic, StraightHR4-315 CrystalWand Magnetic, 45° (McMiken Tool)
ApplicationsCrystalCap Magnetic & CrystalCap Copper Magnetic handling toolDescription
The CrystalWand Magnetic is designed to be used exclusively with the CrystalCap Magnetic, CrystalCap Copper Magnetic, and CrystalCap HT systems during transfer of the caps from the vial to the goniometer head and from the goniometer to the vial. The 6 1/2” (165 mm) long chrome plated steel wand features a plastic housing enclosing a spring tensioned plunger that when depressed, moves a non-magnetic steel platform away from the magnet housed in the end of the wand. This causes the steel CrystalCap to detach from the wand and be placed readily into the vial or the CryoTong™. When the platform is retracted, the wand securely holds the steel CrystalCap Magnetic. The CrystalWand Magnetic 45° (McMiken Tool) offers the same functionality but is bent at a 45° angle at approximately 2.5 cm from the magnet end.HR4-729 CrystalWand Magnetic, StraightHR4-315 CrystalWand Magnetic, 45° (McMiken Tool)
Vial Clamp™ – Curved
ApplicationsVial support & manipulation
FeaturesCurved end tipHemostat style closureDescription
The Vial Clamp – Curved is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems at an angle when the clamp is closed. The clamp can be locked using the hemostat style closure. The end of the clamp, where the vial is held, is curved at either a 45°/135° or 110°/ 70° angle. When the vial is placed in the clamp, the length of it is positioned to the clamp at either of those same angles. The overall length of each clamp is the same, 195 mm.HR4-671 Vial Clamp – Curved 45°/135°HR4-672 Vial Clamp – Curved 110°/70°
ApplicationsVial support & manipulation
FeaturesCurved end tipHemostat style closureDescription
The Vial Clamp – Curved is a chrome plated, hemostat style tool. It has a tip shaped to hold the storage vial of all the CrystalCap Systems at an angle when the clamp is closed. The clamp can be locked using the hemostat style closure. The end of the clamp, where the vial is held, is curved at either a 45°/135° or 110°/ 70° angle. When the vial is placed in the clamp, the length of it is positioned to the clamp at either of those same angles. The overall length of each clamp is the same, 195 mm.HR4-671 Vial Clamp – Curved 45°/135°HR4-672 Vial Clamp – Curved 110°/70°
CrystalCap™ Holder
pplicationsCrystalCap support inside stainless steel dewars
FeaturesAdjustable heightSupports up to 8 CrystalCaps15 ml vial position for liquid propane500 and 1,000 ml versionsDescription
CrystalCap Holders are convenient stands for holding all CrystalCap™ systems in liquid nitrogen dewars during crystal cryogenic procedures. Available in two sizes. Both holders feature spring-clips to secure the CrystalCap Vials in the holder. Both stands have 3 inches of adjustable Z (height) which allows one to raise or lower the CrystalCaps to the appropriate height, depending upon the liquid nitrogen level in the dewar. Each holder is supplied with a socket driver for height adjustment.500 ml version holds five CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 500 ml dewar. 1,000 ml version holds eight CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 1,000 ml dewar.HR4-707 CrystalCap Holder – 500 ml
HR4-706 Replacement Height Adjustment Tool
pplicationsCrystalCap support inside stainless steel dewars
FeaturesAdjustable heightSupports up to 8 CrystalCaps15 ml vial position for liquid propane500 and 1,000 ml versionsDescription
CrystalCap Holders are convenient stands for holding all CrystalCap™ systems in liquid nitrogen dewars during crystal cryogenic procedures. Available in two sizes. Both holders feature spring-clips to secure the CrystalCap Vials in the holder. Both stands have 3 inches of adjustable Z (height) which allows one to raise or lower the CrystalCaps to the appropriate height, depending upon the liquid nitrogen level in the dewar. Each holder is supplied with a socket driver for height adjustment.500 ml version holds five CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 500 ml dewar. 1,000 ml version holds eight CrystalCaps, two 15 ml vials (for liquid propane procedures) and fits into the 1,000 ml dewar.HR4-707 CrystalCap Holder – 500 ml
HR4-706 Replacement Height Adjustment Tool
Hampton蛋白质结晶捞针（结晶环）18 mm Mounted CryoLoop™
Hampton蛋白质结晶捞针（结晶环）18 mm Mounted CryoLoop™
Applications
 |
Cryocrystallography |
Features
|
Adjustable loop orientation | |
|
Synthetic CryoLoop on adjustable stainless steel MicroTube | |
|
MicroTube specially engineered with EasySnap notches | |
|
20 micron diameter nylon CryoLoop |
Description
Similar to the Mounted CryoLoop™ in design and function, the Adjustable Mounted CryoLoop allows one to manipulate and orient the CryoLoop to multiple, unique positions previously unattainable with typical cryo setups. The Adjustable Mounted CryoLoop is a 20 micron diameter synthetic loop mounted inside a malleable stainless steel sleeve, which in turn, is mounted inside the EasySnap MicroTube™.
Using a specially designed L-shaped tool (HR5-902) with a positioning notch, one can manipulate the angle and orientation of the CryoLoop by bending and adjusting the angle of the malleable stainless steel insert which holds the CryoLoop. The Adjustable Mounted CryoLoop may be positioned several times. Note: Repeated manipulation will fatigue the sleeve which can lead to failure of the sleeve. The Adjustable Mounted CryoLoop is designed to work with the CrystalCap System components.
The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and stake it to the CrystalCap™ Magnetic or CrystalCap™ Copper Magnetic. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic.
Most data collection geometries prefer or require a 22 mm length between the base of the cap and the beam. For a 22 mm overall length using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT, snap the MicroTube at the second notch from the bottom, creating an 18 mm MicrozTube lenghth and secure to the CrystalCap using epoxy or similar adhesive. For a 22 mm over length using the CrystalCap Copper, CrystalCap Copper HT, CrystalCap ALS Copper HT snap the MicroTube at the fifth notch from the bottom (notch closest to the CryoLoop) and secure to the CrystalCap using epoxy or similar adhesive.
The HR5-902 Tool for Adjustable Mounted CryoLoop is not included with the Adjustable Mounted CryoLoop and must be purchased as a separated item.
18 mm Mounted CryoLoop
18 mm Mounted CryoLoop properly installed in a CrystalCap Magnetic
Crystal in CryoLoop
| CAT NO | NAME | DESCRIPTION |
| HR4-932 | 18 mm Mounted CryoLoop – 20 micron | 0.025 – 0.05 mm – 25 pack |
| HR4-945 | 18 mm Mounted CryoLoop – 20 micron | 0.05 – 0.1 mm – 25 pack |
| HR4-947 | 18 mm Mounted CryoLoop – 20 micron | 0.1 – 0.2 mm – 25 pack |
| HR4-970 | 18 mm Mounted CryoLoop – 20 micron | 0.2 – 0.3 mm – 25 pack |
| HR4-971 | 18 mm Mounted CryoLoop – 20 micron | 0.3 – 0.4 mm – 25 pack |
| HR4-972 | 18 mm Mounted CryoLoop – 20 micron | 0.4 – 0.5 mm – 25 pack |
| HR4-973 | 18 mm Mounted CryoLoop – 20 micron | 0.5 – 0.7 mm – 25 pack |
| HR4-974 | 18 mm Mounted CryoLoop – 20 micron | 0.7 – 1.0 mm – 25 pack |
| HR4-918 | 18 mm Mounted CryoLoop – 20 micron | Sampler – 30 pack (5 of each diameter size 0.1 – 1.0 mm) |
Hampton蛋白结晶小瓶和结晶柱底座CrystalCap™
Hampton蛋白结晶小瓶和结晶柱底座CrystalCap™
Applications
|
Cryocrystallography 低温结晶学 |
Features
|
SSRL style sample mount for cryocrystallography | |
|
CrystalCap attaches magnetically to CrystalCap Vial and Magnetic Goniometer Base | |
|
Conical shape with ledge compatible with SSRL style grippers, auto mounters and sample handlers |
Description
The CrystalCap is a magnetic sample mount (also known as a cap, pin or goniometer base) designed for cryocrystallography systems that accept SSRL style sample mounts. The CrystalCap attaches to a magnetic CrystalCap Vial and Magnetic Goniometer Base. The tip of the CrystalCap accepts a Mounted CryoLoop™ or MicroTube™ fitted with a CryoLoop™.
The CrystalCap Vial is a 1.8 ml cryo vial featuring two small vents. A ring magnet is molded into the open end of the vial so that when the cap is positioned in the vial, the ring magnet holds the cap on the vial during cryogenic storage.The HR4-904 CrystalCap Vial does not have a magnet on the bottom of the vial.
The conical shape with ledge is compatible with SSRL style grippers, auto mounters and sample handlers.
Compatible with the following Synchrotron Radiation Beamlines
North & South America
• The Advanced Light Source, Berkeley, California ALS 4.2.2, ALS 11.3.1, ALS 12.2.2, ALS 12.3.2
• The Advanced Photon Source, Argonne, Illinois APS 14-BM-C BioCARS, APS 14-ID-B BioCARS
• Cornell High Energy, Synchrotron Source, Ithaca, New York CHESS A1, CHESS F1
• Canadian Light Source, Saskatchewan, Canada, CLS 08ID-1, CLS 08B1-1
• National Synchrotron Light Source, Upton, New York NSLS X4C
• Stanford Synchrotron Radiation Laboratory, Menlo Park, California SSRL BL7-1, SSRL BL9-1, SSRL BL9-2, SSRL BL11-1, SSRL BL12-2, SSRL BL14-1
Asia & Australia
• Shanghai Synchrotron Radiation Facility, Shanghai, China SSRF BL17U1
• National Synchrotron Radiation Research Center, Taiwan NSRRC BL13B1, NSRRC BL13C1
• Pohang Accelerator Laboratory, Pohang, South Korea PAL 2D, PAL 5C, PAL 7A
• Photon Factory, Tsukuba, Japan PF BL-5A, PF BL-17A, PF AR-NW12A
• Super Photon ring-8 GeV, Japan SPRING-8 BL12B2, SPRING-8 BL24XU, SPRING-8 BL26B1, SPRING-8 BL26B2, SPRING-8 BL32B2, SPRING-8 BL32XU, SPRING-8 BL38B1, SPRING-8 BL41XU, SPRING-8 BL44B2, SPRING-8 BL44XU
CrystalCap
CrystalCap & Vial
CrystalCap
Hampton蛋白结晶小瓶和结晶柱底座 CrystalCap™
| CAT NO | NAME | DESCRIPTION |
| HR4-733 | CrystalCap | with Vial – 60 pack |
| HR4-902 | CrystalCap | without Vial – 60 pack |
| HR4-904 | CrystalCap Vial | Vial only – 30 pack |
Hampton晶体删选试剂盒
Hampton晶体删选试剂
1．Screens 晶体删选试剂盒
| 产品名称 | 货号 | 用途备注 |
| Index Kit | HR2-144 | 用于生物大分子结晶 |
| CrystalScreen Kit | HR2-110 | 用于蛋白质，核酸水溶性小分子结晶 |
| Crystal Screen 2 Kit | HR2-112 | |
| Pegrx 1 | HR2-082 | 用于生物大分子结晶 |
| Pegrx 2 | HR2-084 | 用于生物大分子结晶 |
| Peg/Ion Screen Kit | HR2-126 | 用于生物大分子结晶 |
| Peg/Ion 2 Screen™ | HR2-098 | 用于生物大分子结晶 |
| Saltrx Kit | HR2-108 | 用于生物大分子结晶 |
| Membfac Kit | HR2-114 | 用于膜蛋白和溶解度有限的样品结晶 |
| Crystal Screen Lite | HR2-128 | 用于蛋白质，缩氨酸，核酸的结晶 |
| Natrix Kit | HR2-116 | 用于核酸和蛋白核酸复合物的结晶 |
| Crystal Screen Cyro Kit | HR2-122 | 用于蛋白质，可溶性缩氨酸，核酸和水溶性小分子的初次低温结晶 |
| Nucleic Acid Mini Screen Kit | HR2-118 | 用于核酸片段的结晶 |
| Low Ionic Strength Screen | HR2-120 | 用于完整单抗，单抗片段以及在低离子浓度下溶解度较低的样品结晶 |
| Silver Bullet & Silver Bullet Bio | HR2-078 | 用于生物大分子的初次和二次结晶 |
| HR2-080 | ||
| Peg/Tacsimate Ph5.8 Crystallization Reagent | HR2-090 | |
| Peg/Tacsimate Ph 6.8 Crystallization Reagent | HR2-092 | |
| Peg/Tacsimate Ph 7.8 Crystallization Reagent | HR2-094 | |
| Additive Screen™ | HR2-428 | 利用样品间和样品溶剂间相互作用来优化晶体生长和改变样品溶解度 |
| 产品名称 | 货号 | 用途备注 |
| Detergent Screen 1 | HR2-410 | 改善疏水作用引起的非特异性聚集 |
| Detergent Screen 2 | HR2-411 | |
| Detergent Screen 3 | HR2-412 | |
| Detergent Screen | HR2-408 | |
| Heavy Atom Screen Pt | HR2-442 | Heavy Atoms For Multiple Isomorphous Replacement |
| Heavy Atom Screen Au | HR2-444 | |
| Heavy Atom Screen Hg | HR2-446 | |
| Heavy Atom Screen M1 | HR2-448 | |
| Heavy Atom Screen M2 | HR2-450 | |
| I3c Phasing Kit | HR3-133 |
2.Custom Shop Crystallization Reagents 结晶试剂盒中单独的试剂
- Optimize™ Reagents晶体生长条件优化试剂
⑴ Optimize™ – Polymers聚合体
⑵ Optimize™- Organics (Volatile) 挥发性有机物
⑶ Optimize™- Organics (Non-Volatile)非挥发性的有机物
⑷ Optimize™- Salts 盐类
⑸ Optimize™- Buffers缓冲液
⑹ Optimize™- Solubilizing Agents (NDSB) 溶解试剂NDSB
⑺ Optimize™ – Reducing Agent还原剂-TCEP氢氯化物
⑻ Optimize™ – Cryoprotectants
⑼ Optimize™ – Oils用于结晶的油类
⑽ Optimize™ – Silica Hydrogel硅胶
⑾ Optimize™ – LM Agarose低熔点琼脂糖
5.Crystallization Plates, Hardware & Accessories结晶耗材
⑴ 24 Well Crystallization Plates 24孔晶体板-分悬滴和坐滴两种
⑵ 48 Well Crystallization Plates 48孔晶体板
⑶ 72 Well Crystallization Plates72孔晶体板
⑷ 96 Well Crystallization Plates 96孔晶体板等
Hampton 0.1毫米50号玻璃毛细管
Hampton 0.1毫米50号玻璃毛细管
货号：HR6-104
包装：25 pack
- 英文名：0.1 mm Glass Number 50 Capillary
- 品牌：Hampton
Hampton代理–金畔生物
硼硅玻璃50号毛细管壁厚0.01毫米，全长80毫米+ / – 5毫米。提供外径从0.1毫米到2毫米的毛细管，用于X射线数据收集，晶体密度测量和晶体生长实验。毛细管可以用蜡，环氧树脂等密封材料封闭，防止水分和气体。
应用
X射线数据采集
液-液扩散结晶
凝胶针状结晶
特征
50号硼硅玻璃
壁厚 10微米
描述
硼硅玻璃50号毛细管一端呈漏斗形，另一端封闭，壁厚0.01毫米，全长80毫米+/-5毫米，外径从0.1毫米到2毫米。可嵌入，盛放，储存小分子和生物大分子晶体用于X射线数据收集，晶体密度测量和晶体生长实验。毛细管可以用蜡，环氧树脂和其它密封材料密封，防止水分和气体。
请参照“线性吸收系数µcm-1表“，以决定适合的玻璃/石英玻璃毛细管。本表展示的是X射线数据收集时的辐射吸收量。
0.1mm至2.0mm毛细管开口端的基础尺寸为：3.0+/-0.15mm外径 x 0.13+/-0.10 mm壁厚，直径为在毛细管封闭端40毫米处的测量值。
|
货号 |
产品说明 |
公差 |
|
HR6-104 |
Size: 0.1 mm – 25 pack |
0.1 mm -0.075 / +0.075 mm |
|
HR6-106 |
Size: 0.2 mm – 25 pack |
0.2 mm -0.050 / +0.050 mm |
|
HR6-108 |
Size: 0.3 mm – 25 pack |
0.3 mm -0.050 / +0.100 mm |
|
HR6-110 |
Size: 0.4 mm – 25 pack |
0.4 mm -0.100 / +0.100 mm |
|
HR6-112 |
Size: 0.5 mm – 25 pack |
0.5 mm -0.100 / +0.100 mm |
|
HR6-114 |
Size: 0.6 mm – 25 pack |
0.6 mm -0.100 / +0.100 mm |
|
HR6-116 |
Size: 0.7 mm – 25 pack |
0.7 mm -0.150 / +0.150 mm |
|
HR6-118 |
Size: 0.8 mm – 25 pack |
0.8 mm -0.150 / +0.150 mm |
|
HR6-120 |
Size: 0.9 mm – 25 pack |
0.9 mm -0.150 / +0.150 mm |
|
HR6-122 |
Size: 1.0 mm – 25 pack |
1.0 mm -0.150 / +0.250 mm |
|
HR6-124 |
Size: 1.5 mm – 25 pack |
1.5 mm -0.250 / +0.250 mm |
|
HR6-126 |
Size: 2.0 mm – 25 pack |
2.0 mm -0.250 / +0.250 mm |
Hampton 膜蛋白结晶试剂 MembFac • Crystal Screen Lite • MembFac HT
Hampton MembFac • Crystal Screen Lite • MembFac HT
Applications
Primary sparse matrix crystallization screen for membrane proteins and samples with limited solubility 膜蛋白和样品溶解度有限样品
Features
Membrane protein sparse matrix screens
Sparse matrix formula efficiently samples salts, polymers, organics and pH
pH range 4.6 – 8.5
Formulated for use with detergents
Tube or Deep Well block format
Crystal Screen Lite features a lower ionic strength than the original Crystal Screen
Description
MembFac is a highly effective sparse matrix screen specifically designed as a preliminary screen for the crystallization of membrane proteins. MembFac is based upon the highly effective biased sparse matrix methodology. The MembFac screen matrix is optimized for the crystallization of membrane proteins.
MembFac contains 48 unique reagent formulations. With this set of 48 conditions, numerous chemicals and chemical combinations are utilized, allowing one to evaluate a large variety of potential crystallization conditions. MembFac covers a broad pH range between 4 and 9.
The Rationale
The basic rationale of MembFac is to perform a sparse matrix screen while changing the detergent dimension because detergents play an important factor in the crystallization of membrane proteins. A MembFac screen is to be completed for each detergent. Detergent screens are available separately.
The Method
The membrane protein of interest is first isolated in the detergent which gives the highest stability and activity. The protein concentration should be approximately 10 to 20 mg/ml and the detergent concentration should be only slightly above the CMC. The sample is then set against MembFac, using all 48 solutions plus the screening detergent(s) of choice. The screening detergent concentration should be approximately one to three times the CMC. Hence, for each detergent screened, one would utilize approximately 1 to 2 mg of sample.
The MembFac protocol was used to crystallize membrane proteins including SQR, fumerate: quinone oxireductase, LHII, and LHCII.
Crystal Screen Lite is a sparse matrix of trial crystallization reagent conditions based upon the Crystal Screen™.3 The primary screen variables are salt, pH, and precipitant (salts, polymers, volatile organics, and non-volatile organics).2Crystal Screen Lite differs from the original Crystal Screen kit in that the primary precipitant reagents are one-half the concentration of that used in the original Crystal Screen formulation. The secondary salts, ions, and buffers remain at the original Crystal Screen concentration. Reducing the primary concentration of the primary precipitant results in a screen which is “more gentle” on the sample and typically produces much less precipitate conditions than the original Crystal Screen.
MembFac contains 48 unique reagents, 10 ml each.
MembFac HT contains 1 ml each of all 48 reagents from MembFac and reagents 1-48 from Crystal Screen Lite in a single Deep Well block format.
Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
| CAT NO | NAME | DESCRIPTION |
| HR2-114 | MembFac | 10 ml, tube format |
| HR2-128 | Crystal Screen Lite | 10 ml, tube format |
| HR2-137 | MembFac HT | 1 ml, Deep Well block format |
Hampton结晶用缓冲盐试剂盒– SaltRx
Hampton结晶用缓冲盐试剂盒– SaltRx
SaltRx 1 • SaltRx 2 • SaltRx HT
Applications
Primary or secondary, salt and pH matrix crystallization screen for biological macromolecules
Features
| Salt versus pH matrix crystallization screen |
| Samples pH 4 to 9 |
| 22 unique salts versus salt concentration and pH |
| Compatible with microbatch, vapor diffusion, liquid & gel diffusion methods |
| Tube or Deep Well block format |
| Preformulated, ready to screen |
| Developed at Hampton Research |
| All salts and buffers in screen readily available as Optimize™ reagents for reproducing and optimizing crystals |
| Individual reagents available through the Hampton Research Custom Shop |
Description
SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules. Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH. The selection, concentration, and pH of the salts were determined by data mining the BMCD1 and other crystallization databases, crystallization reports in the literature, and internal crystallization trials performed at Hampton Research. Based on this analysis, up to 35% of protein crystallizations involve salt as the primary crystallization reagent. SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt only reagents/conditions from screens such as Index™, Crystal Screen™, and Grid Screen™ produce crystals and when further screening for additional salt conditions or optimization is desired. Since this screen does not contain volatile organics, it is compatible with microbatch, vapor diffusion, liquid and gel diffusion crystallization methods. SaltRx may also be used for dialysis crystallization in conjunction with Dialysis Buttons™.The original SaltRx kit (HR2-108) contained 96 unique reagents, 10 ml each. This kit has been discontinued and converted into two 48 reagent based kits, SaltRx 1 and SaltRx 2, available separately, which when used together are identical to the original SaltRx.SaltRx 1 contains 48 unique reagents, 10 ml each. SaltRx 1 contains reagents 1-48 of the original SaltRx 96 reagent kit.Salt Rx 2 contains 48 unique reagents, 10 ml each. SaltRx 2 contains reagent 49-96 of the original SaltRx 96 reagent kit.SaltRx HT contains the 96 SaltRx 1 and SaltRx 2 reagents in a single Deep Well block format.Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules. Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH. The selection, concentration, and pH of the salts were determined by data mining the BMCD1 and other crystallization databases, crystallization reports in the literature, and internal crystallization trials performed at Hampton Research. Based on this analysis, up to 35% of protein crystallizations involve salt as the primary crystallization reagent. SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt only reagents/conditions from screens such as Index™, Crystal Screen™, and Grid Screen™ produce crystals and when further screening for additional salt conditions or optimization is desired. Since this screen does not contain volatile organics, it is compatible with microbatch, vapor diffusion, liquid and gel diffusion crystallization methods. SaltRx may also be used for dialysis crystallization in conjunction with Dialysis Buttons™.The original SaltRx kit (HR2-108) contained 96 unique reagents, 10 ml each. This kit has been discontinued and converted into two 48 reagent based kits, SaltRx 1 and SaltRx 2, available separately, which when used together are identical to the original SaltRx.SaltRx 1 contains 48 unique reagents, 10 ml each. SaltRx 1 contains reagents 1-48 of the original SaltRx 96 reagent kit.Salt Rx 2 contains 48 unique reagents, 10 ml each. SaltRx 2 contains reagent 49-96 of the original SaltRx 96 reagent kit.SaltRx HT contains the 96 SaltRx 1 and SaltRx 2 reagents in a single Deep Well block format.Ready-to-use reagents are sterile filtered and formulated with ultra-pure Type 1 water, using the highest purity salts, polymers, organics and buffers. Individual reagents are available through the Hampton Research Custom Shop.
Measured pH range of kit is 4.1 to 9.0 at 25°C
Average measured pH of kit is 6.9 at 25°C
Median measured pH of kit is 7.3 at 25°C
Mode measured pH of kit is 7.4 at 25°C
| CAT NO | NAME | DESCRIPTION |
| HR2-136 | SaltRx HT | 1 ml, Deep Well block format |
| HR2-107 | SaltRx 1 | 10 ml, tube format |
| HR2-109 | SaltRx 2 | 10 ml, tube format |
Hampton Polyethylene glycol 4,000
Hampton Polyethylene glycol 4,000
Applications
Crystallization grade Polyethylene glycol for formulating screens or for optimizatio
Features
Sterile filtered solution
Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
Description
Polyethylene glycol 4,000
Synonym: PEG 4,000
H(OCH2CH2)nOH
Mr 3,500-4,500
CAS Number [25322-68-3]
EC Number 500-038-2
Merck 14,7568
RTECS TQ3500000
MDL Number MFCD00081839
PubChem Substance ID 24890217
Melting point (Starting Material) 58.0-61.0°C
Aldehyde None detected
Peroxide None detected
HR2-529 Measured pH range: 6.9 – 9.5 at 25°C
HR2-529 Measured Conductivity Range: 35.3 – 46.9 µS/cm at 25°C
HR2-529 Measured Refractive Index Range: 1.40172 – 1.40279 at 20°C
| CAT NO | NAME | DESCRIPTION |
| HR2-605 | Polyethylene glycol 4,000 | Flake – 500 g |
| HR2-529 | Polyethylene glycol 4,000 | 50% w/v solution – 200 ml |
Hampton Solubility & Stability Screen
Hampton Solubility & Stability Screen
Solubility & Stability Screen（溶解度和稳定性的屏幕）
应用：溶解性和稳定性屏幕的设计，以协助识别解决方案的条件，促进蛋白质的溶解性和稳定性，并尽量减少蛋白质沉淀。
Solubility & Stability Screen
Solubility & Stability Screen HR2-072
Applications
| A solubility screen, a stability screen, an additive screen for protein assays including ThermoFluor and crystallization |
| Preformulation screening |
| Compatible with Thermofluor , Differential Scanning Fluorimetry (DSF), and Protein Thermal Shift assays |
| 96 sterile filtered reagents |
| 17 classes of reagents and excipients |
| Highly concentrated (10x) reagent formulation |
| Developed at Hampton Research |
Features
Methodologies
| Thermal shift assay |
| Dynamic Light Scattering |
| Use Solubility & Stability Screen along side Slice pH and Solubility & Stability Screen 2 to optimize sample buffer |
Description
Solubility & Stability Screen is designed to assist in the identification of solution conditions which promote protein solubility and stability, and minimize protein precipitation. Solubility & Stability Screen is a solubility screen, a stability screen, and may also be used as an additive screen in the presence of a crystallization reagent.
The Hampton Research Solubility and Stability Screen can evaluate protein solubility, stability and crystallization in the presence of 94 different chemical additives sampling 17 different classes of reagents plus two controls.
Protein solubility and stability are universally required in a wide range of applications, including general biochemical studies, the preparation of proteins in pharmaceuticals, structural biology and crystallization.1-11The preparation of a concentrated, soluble and stable protein sample can often be a difficult task as proteins often aggregate, precipitate or denature.
Thermofluor (also known as Differential Scanning Fluorimetry, DSF and Protein Thermal Shift) provides a rapid, convenient and effective means of identifying subsaturating solution conditions that may also correlate with protein heterogeneity and poor crystallization outcomes.12
Protein solubility and stability is affected by many different chemical factors including pH, buffer type, chemical additives and excipients. pH and buffer type are dominant protein solubility and stability variables and can be evaluated and optimized using the Hampton Research Slice pH kit HR2-070. Slice pH evaluates protein solubility, stability and crystallization versus 20 different buffers over the pH range 3.5-9.6. Chemical additives influencing protein solubility and stability can be evaluated using the Solubility & Stability Screen.
It is widely accepted that protein solubility and stability can be increased by the use of chemical additives.5,15Seventeen classes of reagents are sampled by the Solubility & Stability Screen and each of these classes has been reported as important in improving sample solubility and stability.2-11
The Solubility & Stability Screen is a set of 94 high purity reagents formulated in high purity water (NCCLS/ASTM Type 1+) at 25°C and are 0.22 micron sterile filtered. The 94 solubility and stability reagents are formulated at 2 to 10 times their recommended working concentration. The remaining two reagents are water and a negative (TCA) control. A water control demonstrates the effect of diluting sample as well as sample buffer concentration. TCA, the negative control, demonstrates total sample precipitation, loss of sample solubility and loss of sample stability. The effects of the Solubility & Stability reagents can be compared with this negative control to assist in the identification of reagents promoting sample solubility and stability. 500 microliters of each reagent is supplied in a sterile 96 well polypropylene Deep Well block. The Solubility & Stability Screen reagents are compatible with the sitting or hanging drop vapor diffusion, microbatch, free interface diffusion, sandwich drop vapor diffusion, and dialysis crystallization methods utilizing water soluble reagents.